Microwave-Assisted Synthesis of Pd Nanoparticles and Catalysis Application for Suzuki Coupling Reactions

A facile and efficient method was developed for the synthesis of highly active palladium nanoparticles (PdNPs) by microwave-assisted chemical reduction. The PdNPs with different morphology were prepared with or without KBr (capping agent) using ethylene glycol (EG) and citric acid (CA) as reducing agent in the presence of polyvinylpyrrolidone (PVP), respectively. The as-prepared PdNPs were characterized by (UV-Visible Spectrophotometer) UV-Vis, transmission electron microscopy (TEM) and Xray diffraction (XRD) analysis. The results showed that the PdNPs reduced by EG without KBr (PdNPs-EG) has smaller particle size than other PdNPs. The remarkable catalytic activity of the PdNPs catalysts are obtained using a low amount of PdNPs (0.1 mmol‰) using K2CO3 as base and EtOH/H2O as solvent toward Suzuki coupling reactions of aryl bromides and phenylboronic acid for 15 min.


INTRODUCTION
The Suzuki coupling reactions catalyzed by the transition metal palladium are very powerful tools for the construction of C-C bonds since its discovery in 1979 [1].It has been immensely applied to synthesize biphenyl compounds including natural products, agrochemicals, optical materials, and pharmaceuticals due to the mild reaction conditions and the broad functional group tolerance of this transformation [2 -6].
The palladium-based catalysts are considered as the most available coupling catalysts, numerous efforts have been conducted to explore highly active catalytic systems [7].The homogenous Pd catalysts with ligand generally exhibited higher catalytic activity and have been most frequently applied in Suzuki coupling reactions [8].The ligand-free heterogeneous Pd catalysts via supporting Pd on organic polymers or inorganic solids [9 -15] have also achieved much attention due to its recycling and reusing.Recently, Pd nanoparticles (PdNPs) with high surface-to-volume ratio and highly active surface atoms in comparison with those of the bulk catalysts exhibit excellent activity [16] and have attracted great interest.There are many factors such as nanoparticles size and shape that affect the catalytic activity of Pd nanoparticles.Therefore, numerous efforts have been made to develop effective and facile synthetic method to prepare well-defined and uniform-sized metal nanoparticles [17].Microwave irradiation (MWI) methods provide simple and fast routes to the synthesis of nanomaterials without high temperature or high pressure.Moreover, the metal nanoparticles prepared by MWI have nanometer size with narrow size distribution [18 -21].
In this paper, the microwave-assisted reduction method was employed to synthesize the PdNPs using CA and EG as reduction agent, respectively.The catalytic properties of the as-prepared PdNPs for the Suzuki coupling reactions were examined.

Materials and Methods
All chemical reagents used in this experiment including sodium chloride (NaCl), palladium chloride(PdCl 2 ), Polyvinyl Pyrrolidone (PVP), ethanol, potassium bromide (KBr), ethylene glycol and citric acid (CA) were purchased from Tianjin Kermel and Sinopharm Chemical Reagent Co. Ltd.All chemicals were used as received and without further purification.
Transmission electron microscope (TEM) was performed on an FEI Tecnai G2 S-TWIN to obtain TEM images.The X-ray diffraction (XRD) patterns were measured using a Bruker D8 advance diffractometer with a Cu Kα (λ=0.15418nm) radiation at 40 kV and 40 mA.UV-Vis spectrophotometers were obtained with a Shimadzu UV-2450, using a quartz cuvette as sample-holder.

Preparation of PdNPs Reduced by Ethylene Glycol (EG)
Add 10 ml aqueous solution of PVP (0.5 mol/L) to 10 ml aqueous solution of Na 2 PdCl 4 (0.05 mol/L) in a 50 ml beaker and the solutions were stirred for 30 min followed by the addition of 2 ml ethylene glycol and 8 ml deionized water at room temperature.The mixture was then reduced by microwave irritation method at 85°C for a total reaction time of 90 min after the solutions were stirred for another 30 min.After the reaction, a black stable suspension of Pd nanoparticles was obtained.The as-prepared PdNPs was denoted as PdNPs-EG.In addition, the PdNPs-KBr-EG was prepared following the same procedure mentioned above except for adding 0.2380 g KBr to the solution of Na 2 PdCl 4 and PVP.

Preparation of PdNPs Reduced by Citric Acid (CA)
The same procedure was used for the preparation of PdNPs except that the 10 ml citric acid aqueous solution (0.2 mol/L) was slowly added as the reducing agent and the mixture was subjected to microwave irritation for 120 min at 90°C.After the reaction, the obtained PdNPs was named as PdNPs-CA.The PdNPs-KBr-CA was also synthesized by dissolving 0.2380 g KBr in the solution of Na 2 PdCl 4 and PVP before adding citric acid.

General Procedure for Suzuki Reactions
Suzuki coupling reactions were conducted as follows: aryl halide (1 mmol), phenylboronic acid (1.5 mmol) and potassium carbonate (2 mmol) were dissolved in a mixture solvent of 12 ml ethanol:water (v/v = 1/1) and placed in a 30 ml microwave tube.The PdNPs were then added, and the tube was sealed and treated by microwave irradiation at 60°C.The amount of PdNPs and reaction time were recorded in Table 1.
Upon reaction completion, 5 ml sodium hydroxide solution (0.2 mol/L) was added to the reaction mixture (5 ml), and the products were extracted with ethyl acetate (10 ml).The organic layers were combined, dried in air to give a solid product.Analysis of the reaction products was carried out by the high performance liquid chromatography (HPLC) UltiMate 3000 using a mobile phase consisting of methanol and H 2 O (v/v=90:10).Column temperature was set to 35°C.UV-detection was performed at wavelengths of 254 nm over a run time of 15 min.

Catalysts Characterization
The formation of PdNPs-EG and PNPs-CA are monitored by UV-Vis spectrum, respectively.The progression of PNPs reduced by EG is shown in Fig. (1a).There is a strong characteristic peak around 420 nm in the spectrum in the absence of EG, which is attributed to Pd (II).It is obviously observed that the intense of Pd (II) peak gradually become weak after mixing with EG and treating by microwave irradiation at 85°C.At the same time, the color of the mixture turns from yellow to black indicating the formation of PdNPs.The Pd (II) peak totally disappears after microwave irritation for 90 min indicating the complete reduction of Pd (II) in the mixture.From the Fig. (1b), the same phenomenon is found for the reduction process of PdNPs-CA except that the Pd (II) peak completely disappears after microwave irritation for 120 min at 90°C.The XRD patterns of the as-prepared PdNPs-EG, PdNPs-KBr-EG, PdNPs-CA and PdNPs-KBr-CA are shown in Fig. (2).Three main diffraction peaks with 2θ of 40.1°, 46.6°, 68.1° are observed for all samples which are ascribed to Pd (111), Pd (200), Pd (220) phases, respectively, which match well with the standard face centered cubic (fcc) structure according to the JCPDS card no.46-1043.For the PdNPs-KBr-EG, the peaks are stronger and narrower than those of the PdNPs-EG indicating the larger average particle size and higher crystallinity.Otherwise, the stronger and slight broader peaks are found in PdNPs-KBr-CA compared to the PdNPs-CA.
The TEM images and particles size distribution of as-prepared PdNPs are displayed in Fig. (3).From the images, the Pd nanoparticles with different morphology are all highly distributed in system indicating the microwave-assisted method can improve the dispersion of PdNPs in an aqueous phase.Figs.(3a and b) show the TEM images of the Pd nanoparticles reduced by EG with or without KBr, respectively.TEM image Fig. (3a) shows the occurrence of highly dispersed spherical PdNPs with a narrow and homogeneous distribution.The particle size is range from 2.59-5.42nm with an average diameter of 3.74 nm.The rod-shaped PdNPs with a larger particle size between 3.25-9.78nm (average diameter of 6.62 nm) than PdNPs-EG are prepared in the presence of KBr.The TEM images of the Pd nanoparticles reduced by CA with or without KBr are presented in Figs.(3c and d), respectively.The PdNPs-CA of different shape such as cube, tetrahedron and other polyhedron with an average diameter of 8.91 nm are found.TEM observation of PdNPs-KBr-CA shows a cubic and rodlike shape with an average particle size of 8.05 nm indicating an obvious different from the shape of PdNPs-CA, but a similar shape to PdNPs-KBr-EG.A possible reason for the change of PdNPs shapes is that bromide can absorb on the surface of PdNPs and change the order of surface free energies for different facets to promote the formation of different facets.Pd nanoparticles with cube and rod shape are easier to synthesize in the presence of KBr.

Catalytic Activity Toward Suzuki Reaction
The catalytic activity of PdNPs-EG, PdNPs-KBr-EG, PdNPs-CA and PdNPs-KBr-CA were evaluated against the Suzuki coupling reaction by microwave irritation.We chose 4-bromotoluene and phenylboronic acid as a model reaction substrate (Scheme 1) to study the impact of various reaction conditions such as base and solvent on the reaction.Scheme 1. PdNPs catalysts for Suzuki coupling reaction of 4-bromotoluene and phenylboronic acid.
Screening with different solvents shows that ethanol is a more effective solvent for this system; the solvent, MeOH gives slightly lower yields of 96.9%, 95.9%, 95.2% and 95.7% for PdNPs-EG, PdNPs-KBr-EG, PdNPs-CA and PdNPs-KBr-CA, respectively (Table 1, entry 6).Poor yields of the product are obtained from other solvents (Table 1, entries 7-11).Adopting a green chemical approach, nontoxic ethanol is cheap and practical for use as a solvent for the synthesis.
To evaluate the catalytic ability of the prepared PdNPs catalysts, the Suzuki coupling reactions of phenylboronic acid and a diverse range of aryl halides were carried out using microwave irradiation.As illustrated in Table 2, the 4-OCH 3 , 4-NO 2 , 4-COCH 3 , 4-CN and 4-OCH 3 substituted aryl bromides obtain high yields of the corresponding product in the presence of 0.1 mmol‰ for 15 min.For instance, the yield of 99.3%, 98.8%, 97.8% and 98.2% are obtained for the Suzuki coupling reaction of 4-bromoacetophenone with phenylboronic acid catalyzed by PdNPs-EG, PdNPs-KBr-EG, PdNPs-CA and PdNPs-KBr-CA, respectively (Table 2, entry 4).However, because of the steric hindrance effect, the reactions of o-bromotoluene with phenylboronic acid provide a lower yield than the results for the reactions of pbromotoluene with phenylboronic acid under the same reaction conditions (Table 2, entries 2 vs 7).Especially for the obromoanisole, the poor yields (less than 50%) are provided even though prolonging reaction time to 30 min (Table 2, entry 8).The Suzuki coupling reactions between phenylboronic acid and aryl chloride in the presence of 1 mmol‰ PdNPs were also investigated.As shown in Table 2, the very low yields are obtained under the current reaction conditions and a moderate yield is provided for the reaction of p-chloroacetophenone with phenylboronic acid catalyzed by PdNPs-EG (Table 2, entries 9-11).Additionally, the moderate yields are obtained for the reactions of phenylboronic acid with heteroaryl bromide using 1 mmol‰ PdNPs (Table 2, entries 12-14).Considering the above results, the PdNPs-EG with smaller particle size and regular shape obtains a higher catalytic activity compared with PdNPs-KBr-EG, PdNPs-CA and PdNPs-KBr-CA by comparing the yields for the same substrates under the same reaction conditions.

CONCLUSION
In conclusion, the PdNPs with small particle size and high degree of dispersion protected by PVP have been prepared using ethylene glycol (EG) and citric acid (CA) as reducing agent, PVP as protecting agent by microwaveassisted chemical reduction.The Pd nanoparticles of special shape with an average diameter of 3.74 nm and heterogeneous shape with an average diameter of 6.62 nm are synthesized by EG and CA in the absence of KBr, respectively.The PdNPs of cubic and rodlike shape are formed with KBr as a capping agent.The prepared PdNPs provided high yields in the Suzuki coupling reactions of various aryl bromides and phenylboronic acid using a low amount of PdNPs (0.1 mmol‰) in a short reaction time.A 98.4% yield is obtained for the Suzuki coupling reaction of 4-bromotoluene and phenylboronic acid catalyzed by PdNPs-EG.The 45.6% and 88.9% yields are obtained for Suzuki coupling reactions of phenylboronic acid with p-chloroacetophenone and 2-bromopyridine catalyzed by the PdNPs-EG at a low Pd loading (1mmol‰) for 6 h, respectively.

Table 1 . The effect of base and solvent on the Suzuki coupling reaction a .
b PdNPs-EG c PdNPs-KBr-EG d PdNPs-CA e PdNPs-KBr-CA.